Problem
Integrating multiple conventional DNA/RNA analysis methods (e.g., DNA/RNA extraction, purification, elution, and amplification) into microfluidic chips can easily lead to exceedingly large, costly, and power-hungry systems. For example, conventional thermal cycling requires special care and additional “pre-PCR” isolation rooms to avoid contamination from previous assays. Similarly, magnetic beads are often used to wash or isolate nucleic acids which may require large, power-hungry electromagnetic systems to immobilize the beads, creating excessive heat emissions.
Solution
Combine various DNA/RNA analysis methods on a single chip and use simple microfluidic handling features to drive and move fluids accurately. This approach minimizes device footprint, power consumption, complexity, cross-contamination, and equipment cost. In this example, a handheld microfluidics drive system (including embedded magnets) purifies nucleic acids with beads without generating excessive heat. The device will drive the purified DNA/RNA directly to an on-chip thermal chamber, thus confining the entire amplicon within one chip and reducing the chance of cross-contamination.
Specifications
Useable Materials for the Plastic Chip:
- COC
- COP
Example Applications
- DNA/RNA purification
- Sample prep / Library prep
- PCR
- NGS
- Other molecular tests and research